TRPM5抗体,Rabbit Polyclonal TRPM5 Antibody
  • TRPM5抗体,Rabbit Polyclonal TRPM5 Antibody
  • TRPM5抗体,Rabbit Polyclonal TRPM5 Antibody
  • TRPM5抗体,Rabbit Polyclonal TRPM5 Antibody

TRPM5抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-09
QQ交谈 微信洽谈

产品详情

中文名称:TRPM5抗体英文名称:Rabbit Polyclonal TRPM5 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 230 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: TRPM5
2025-05-09 TRPM5抗体 Rabbit Polyclonal TRPM5 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 230 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/200 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/3000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesMTR1; LTRPC5
WB Predicted band size131 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human TRPM5
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Mouse heart tissue, Primary antibody: P04505(TRPM5 Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    


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       The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P04505(TRPM5 Antibody) at dilution 1/60. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P04505(TRPM5 Antibody) at dilution 1/60. (Original magnification: ×200)    


           

参考文献

以下是关于TRPM5抗体的3篇参考文献示例(内容基于真实研究领域,但具体文献为模拟概括):

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1. **文献名称**: "TRPM5-dependent calcium signaling in taste receptor cells regulates gustatory perception"

**作者**: Zhang Y, Hoon MA, Chandrashekar J, et al.

**摘要**: 该研究利用TRPM5特异性抗体,通过免疫组化和Western blot技术,证实TRPM5在味觉受体细胞中的高表达。实验表明,TRPM5通过介导钙离子内流参与甜味和苦味信号传导,敲除小鼠模型显示味觉感知显著受损。

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2. **文献名称**: "TRPM5 in pancreatic β-cells: A modulator of glucose-stimulated insulin secretion"

**作者**: Pérez CA, Margolskee RF, Huang L.

**摘要**: 研究使用TRPM5抗体检测其在胰腺β细胞中的表达,发现TRPM5通过调控细胞内钙振荡影响葡萄糖依赖性胰岛素分泌。抗体阻断实验进一步验证了其在代谢调控中的关键作用,提示其与2型糖尿病病理相关。

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3. **文献名称**: "TRPM5 channels and neuroinflammatory responses in the gut-brain axis"

**作者**: Lee N, Kim S, Rhyu MR.

**摘要**: 通过TRPM5抗体标记,研究揭示了肠道内分泌细胞中TRPM5的表达与迷走神经信号传导的关联。实验表明,TRPM5激活可促进炎症因子释放,提示其在肠-脑轴神经炎症中的潜在调控机制。

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4. **文献名称**: "Structural insights into TRPM5 activation by antibody-guided localization"

**作者**: Smith TJ, Clapham DE, Julius D.

**摘要**: 利用高特异性TRPM5抗体进行冷冻电镜成像,解析了TRPM5蛋白的跨膜结构域构象变化。研究发现,钙离子结合位点与通道开放状态的动态关联,为靶向TRPM5的药物设计提供了结构基础。

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注:以上文献为领域典型研究方向模拟,实际引用需通过PubMed等平台检索具体论文。

       

背景信息

TRPM5 (Transient Receptor Potential Cation Channel Subfamily M Member 5) is a calcium-activated, non-selective cation channel critical in mediating sensory signaling, particularly in taste transduction. It is predominantly expressed in taste receptor cells, where it couples G protein-coupled receptor (GPCR) activation to membrane depolarization, enabling signal transmission for sweet, bitter, and umami tastes. TRPM5 is also found in other tissues, including the gut, respiratory epithelium, and pancreatic β-cells, implicating roles in metabolic regulation and chemosensation.

TRPM5 antibodies are essential tools for studying its expression, localization, and function. These antibodies typically target specific epitopes, such as intracellular N- or C-terminal domains, and are validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Researchers use TRPM5 antibodies to investigate its involvement in diseases like obesity, diabetes, and respiratory disorders, as TRPM5 knockout models show altered glucose homeostasis and taste perception. Additionally, TRPM5's temperature and voltage sensitivity make it a focus in neurobiology and pharmacology studies. Reliable antibodies help elucidate its modulation by signaling molecules (e.g., PLC pathways) and potential as a therapeutic target. Validation via knockout controls or peptide blocking ensures specificity, which is crucial given structural similarities within the TRP channel family.

       
关键字: TRPM5抗体;TRPM5;TRPM5 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
普通会员
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
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