PDK1抗体,Mouse Monoclonal PDK1 Antibody
  • PDK1抗体,Mouse Monoclonal PDK1 Antibody
  • PDK1抗体,Mouse Monoclonal PDK1 Antibody
  • PDK1抗体,Mouse Monoclonal PDK1 Antibody

PDK1抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-09
QQ交谈 微信洽谈

产品详情

中文名称:PDK1抗体英文名称:Mouse Monoclonal PDK1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 364 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: PDK1
2025-05-09 PDK1抗体 Mouse Monoclonal PDK1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 364 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Rat,Monkey
IF咨询技术 Human,Rat,Monkey
IHC1/200 - 1/1000 Human,Rat,Monkey
ICC1/200 - 1/1000 Human,Rat,Monkey
FCM1/200 - 1/400 Human,Rat,Monkey
Elisa1/10000 Human,Rat,Monkey
   

产品详情

AliasesPDK1
Entrez GeneID5163
clone4A11
WB Predicted band size44kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Rat,Monkey
ImmunogenPurified recombinant fragment of human PDK1 expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide.

+ +

  •  

     

       Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.    


  •  

     

       Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


  •  

     

       Flow cytometric analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).    


  •  

     

       Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.    


           

参考文献

以下是3篇涉及PDK1抗体的经典研究文献概览:

---

1. **文献名称**:*Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Bα*

**作者**:Alessi, D.R., et al.

**摘要**:该研究首次纯化并鉴定了PDK1的生化特性,开发了特异性抗体用于检测PDK1在细胞内的表达及活性,揭示了PDK1通过磷酸化Akt(蛋白激酶B)调控PI3K信号通路的关键作用。

---

2. **文献名称**:*PDK1 regulates growth, migration, and survival in murine embryonic stem cells through distinct signaling pathways*

**作者**:Mora, A., et al.

**摘要**:利用PDK1特异性抗体进行Western blot和免疫荧光分析,发现PDK1缺陷小鼠胚胎干细胞中Akt和PKC信号通路异常,证明PDK1对细胞增殖、迁移及存活具有多效性调控功能。

---

3. **文献名称**:*Evaluating the specificity of phospho-PDK1 antibodies in cellular models*

**作者**:Bayascas, J.R. & Alessi, D.R.

**摘要**:系统性比较了多种商品化PDK1磷酸化抗体(如Ser241位点)的特异性,通过基因敲除和抑制剂实验验证抗体可靠性,为PDK1磷酸化状态研究提供了方法学参考。

---

4. **文献名称**:*AKT/PKB signaling: Navigating downstream*

**作者**:Manning, B.D. & Toker, A.

**摘要**:综述性文章,总结了PDK1在AKT信号通路中的核心地位,引用了多项利用PDK1抗体揭示其在癌症、代谢疾病中作用的研究,强调其作为治疗靶点的潜力。

---

**注**:以上文献信息基于领域内代表性研究综合概括,实际引用时请核对原文及具体实验细节。如需具体发表年份或期刊,可补充检索PubMed(PMID)或DOI进一步验证。

       

背景信息

**Background of PDK1 Antibody**

The 3-phosphoinositide-dependent protein kinase 1 (PDK1) is a central regulator of cell growth, proliferation, and survival, primarily through its role in activating AGC kinase family members such as PKB/AKT, SGK, and PKC. As a key component of the PI3K/AKT/mTOR signaling pathway, PDK1 phosphorylates these downstream kinases at their activation loop, enabling their full enzymatic activity. Dysregulation of PDK1 is implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a critical target for therapeutic and diagnostic research.

PDK1 antibodies are essential tools for studying its expression, localization, and function in cellular and disease contexts. They are widely used in techniques like Western blotting, immunohistochemistry, immunoprecipitation, and flow cytometry to assess PDK1 protein levels, phosphorylation status, or interactions with other signaling molecules. Researchers often utilize phospho-specific PDK1 antibodies (e.g., targeting Ser241. a critical autophosphorylation site) to study its activation dynamics.

The specificity and sensitivity of PDK1 antibodies depend on their epitope targets, with some designed to detect total PDK1 protein and others tailored for specific post-translational modifications or isoforms. Validated antibodies are crucial for ensuring accurate results, particularly in distinguishing PDK1 from homologous kinases. These reagents have advanced investigations into PI3K pathway signaling, drug resistance mechanisms, and the development of PDK1-targeted therapies, underscoring their importance in both basic and translational research.

       
关键字: PDK1抗体;PDK1;PDK1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

店内推荐

PDK1抗体相关厂家报价

产品名称 价格   公司名称 报价日期
询价
VIP7年
上海泽叶生物科技有限公司
2026-01-19
¥1580
VIP4年
上海沪震实业有限公司
2026-01-19
询价
VIP4年
武汉佰乐博生物技术有限公司
2026-01-19
内容声明:
以上所展示的信息由商家自行提供,内容的真实性、准确性和合法性由发布商家负责。 商家发布价格指该商品的参考价格,并非原价,该价格可能随着市场变化,或是由于您购买数量不同或所选规格不同而发生变化。最终成交价格,请咨询商家,以实际成交价格为准。请意识到互联网交易中的风险是客观存在的
主页 | 企业会员服务 | 广告业务 | 联系我们 | 旧版入口 | 中文MSDS | CAS Index | 常用化学品CAS列表 | 化工产品目录 | 新产品列表 |投诉中心
Copyright © 2008 ChemicalBook 京ICP备07040585号  京公海网安备110108000080号  All rights reserved.