PBK抗体,Mouse Monoclonal PBK Antibody
  • PBK抗体,Mouse Monoclonal PBK Antibody
  • PBK抗体,Mouse Monoclonal PBK Antibody
  • PBK抗体,Mouse Monoclonal PBK Antibody

PBK抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-10
QQ交谈 微信洽谈

产品详情

中文名称:PBK抗体英文名称:Mouse Monoclonal PBK Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 484 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: PBK
2025-05-10 PBK抗体 Mouse Monoclonal PBK Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 484 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesSPK; CT84; TOPK; Nori-3
Entrez GeneID55872
clone2C8
WB Predicted band size36kDa
Host/IsotypeMouse IgG2b
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human PBK expressed in E. Coli.  
FormulationAscitic fluid containing 0.03% sodium azide.

+ +

  •  

     

       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


  •  

     

       Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.    


  •  

     

       Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


  •  

     

       Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).    


  •  

     

       Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using PBK mouse mAb with DAB staining.    


  •  

     

       Figure5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using PBK mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于PBK抗体的虚构参考文献示例(基于学术文献常见结构,非真实论文):

---

1. **文献名称**: *PDZ-Binding Kinase (PBK) as a Novel Biomarker in Glioblastoma: Antibody Validation and Clinical Implications*

**作者**: Yamada S, et al. (2012)

**摘要**: 本研究通过开发高特异性兔源多克隆PBK抗体,验证其在胶质母细胞瘤组织中的表达水平。免疫组化分析显示,PBK在肿瘤细胞核内显著高表达,且与患者生存率负相关。抗体特异性经Western blot和siRNA敲除实验确认,为PBK作为诊断标志物提供了依据。

2. **文献名称**: *A Monoclonal Antibody Targeting PBK Suppresses Tumor Growth in Colorectal Cancer Models*

**作者**: Huang L, Chen W. (2015)

**摘要**: 研究团队开发了一种靶向PBK激酶结构域的小鼠单克隆抗体(mAb-PBK03),并在结直肠癌移植瘤模型中测试其疗效。实验表明,该抗体通过阻断PBK与下游信号蛋白相互作用,抑制肿瘤细胞增殖并诱导凋亡。抗体特异性通过流式细胞术和免疫沉淀验证。

3. **文献名称**: *PBK/TOPK Antibody-Based Detection of DNA Damage Response in Breast Cancer Stem Cells*

**作者**: Smith J, et al. (2018)

**摘要**: 利用商业化PBK抗体(货号#AB-789X),本研究揭示了PBK在乳腺癌干细胞(BCSCs)DNA损伤修复中的关键作用。免疫荧光显示,PBK在辐射后定位于γH2AX阳性病灶,提示其参与同源重组修复。抗体阻断实验进一步证实PBK功能缺失会增强BCSCs对放疗的敏感性。

---

**注**:以上文献为示例性质,作者与年份均为虚构。实际研究中建议通过PubMed或Google Scholar以“PBK antibody”或“PBK/TOPK”为关键词检索真实文献。

       

背景信息

PBK (PDZ-binding kinase), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase belonging to the mitogen-activated protein kinase (MAPK) family. Discovered in 2000. PBK plays critical roles in cell cycle regulation, DNA damage response, and tumorigenesis. It interacts with key signaling pathways, including the p53 and PLK1 networks, influencing processes like cytokinesis, apoptosis, and cell proliferation. Structurally, PBK contains an N-terminal catalytic domain and a C-terminal PDZ-binding motif that mediates protein-protein interactions.

PBK is predominantly overexpressed in various cancers (e.g., breast, lung, glioblastoma) and correlates with poor prognosis, making it a potential therapeutic target. Its aberrant activation promotes tumor growth, metastasis, and therapy resistance by enhancing DNA repair and survival mechanisms. PBK antibodies are essential tools for detecting PBK expression in research and diagnostics, particularly in immunohistochemistry (IHC), Western blotting, and flow cytometry. They help elucidate PBK's functional roles, validate knockout models, and assess its clinical relevance in cancer progression. Recent studies also explore PBK inhibitors in combination therapies to sensitize cancer cells to chemo- or radiotherapy. However, PBK's dual roles in normal physiology (e.g., embryonic development, immune regulation) necessitate careful evaluation of therapeutic strategies targeting this kinase.

       
关键字: PBK抗体;PBK;PBK Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

店内推荐

PBK抗体相关厂家报价

产品名称 价格   公司名称 报价日期
¥1280
VIP7年
上海泽叶生物科技有限公司
2026-01-07
询价
VIP1年
陕西缔都医药有限责任公司
2025-12-31
¥1950
VIP4年
上海沪震实业有限公司
2026-01-07
内容声明:
以上所展示的信息由商家自行提供,内容的真实性、准确性和合法性由发布商家负责。 商家发布价格指该商品的参考价格,并非原价,该价格可能随着市场变化,或是由于您购买数量不同或所选规格不同而发生变化。最终成交价格,请咨询商家,以实际成交价格为准。请意识到互联网交易中的风险是客观存在的
主页 | 企业会员服务 | 广告业务 | 联系我们 | 旧版入口 | 中文MSDS | CAS Index | 常用化学品CAS列表 | 化工产品目录 | 新产品列表 |投诉中心
Copyright © 2008 ChemicalBook 京ICP备07040585号  京公海网安备110108000080号  All rights reserved.