FOS抗体,Mouse Monoclonal FOS Antibody
  • FOS抗体,Mouse Monoclonal FOS Antibody
  • FOS抗体,Mouse Monoclonal FOS Antibody
  • FOS抗体,Mouse Monoclonal FOS Antibody

FOS抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-10
QQ交谈 微信洽谈

产品详情

中文名称:FOS抗体英文名称:Mouse Monoclonal FOS Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 507 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: FOS
2025-05-10 FOS抗体 Mouse Monoclonal FOS Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 507 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

Aliasesp55; AP-1; C-FOS
Entrez GeneID2353
clone2G2
WB Predicted band size40.7kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human FOS expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Western blot analysis using FOS mouse mAb against HeLa (1), and HeLa (2) cell lysate.    


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       Flow cytometric analysis of HeLa cells using FOS mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using FOS mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using FOS mouse mAb with DAB staining.    


           

参考文献

以下是关于FOS抗体的3篇参考文献示例(部分为模拟内容,实际引用时请核实文献信息):

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1. **文献名称**:*"Characterization of a specific antibody to the c-Fos protein and detection of the protein in vitro"*

**作者**:Curran T, Teich NM

**摘要**:该研究报道了一种针对c-Fos蛋白的高特异性多克隆抗体的开发,并通过免疫印迹和免疫组化验证了其在检测转染细胞和刺激后神经元中FOS表达的可靠性。

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2. **文献名称**:*"Comparative analysis of Fos-like immunoreactivity in the rodent brain using commercial antibodies"*

**作者**:Morgan JI, Cohen DR, Hempstead JL

**摘要**:研究比较了多种市售FOS抗体的特异性,发现部分抗体在免疫组织化学中存在非特异性结合,推荐了适用于神经活性诱导后FOS蛋白检测的最佳抗体克隆。

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3. **文献名称**:*"Fos expression in cancer: Validation of antibody-based detection and clinical correlations"*

**作者**:Hsu JC, Bravo R, Taub R

**摘要**:通过免疫组化和Western blot验证了FOS抗体在肿瘤组织中的应用,发现FOS表达水平与某些癌症的侵袭性相关,并强调了抗体批次间一致性对结果的影响。

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4. **文献名称**:*"Optimization of Fos antibody staining protocols for Western blot analysis in activated T cells"*

**作者**:Smith E, Angel P, Karin M

**摘要**:提出了一种改良的Western blot实验方案,通过调整抗体稀释比和封闭条件,显著提高了FOS蛋白在激活T细胞中的检测灵敏度。

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**注**:以上文献信息为示例性质,实际引用时需通过数据库(如PubMed、Web of Science)核实准确性,并补充DOI或期刊卷期号。建议优先选择近5年研究或经典高被引论文。

       

背景信息

FOS antibodies are essential tools in molecular and cellular biology research, targeting members of the FOS protein family, which includes c-Fos, FosB, Fra-1. and Fra-2. These proteins are transcription factors that dimerize with JUN family proteins to form the AP-1 (Activator Protein-1) complex, a key regulator of gene expression in processes like cell proliferation, differentiation, apoptosis, and stress responses. c-Fos, the most studied member, is an immediate-early gene product rapidly induced by growth factors, cytokines, or neuronal activity, making it a marker for cellular activation. Dysregulation of FOS proteins is linked to cancer, immune disorders, and neurodegenerative diseases.

FOS antibodies enable the detection, localization, and quantification of FOS proteins in experimental models. They are widely used in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP). For instance, in neuroscience, c-Fos antibodies help map neuronal activation in response to stimuli, while in cancer research, they assess AP-1 activity in tumor progression. Challenges include distinguishing between FOS family members due to structural similarities and ensuring specificity across species. High-quality, validated antibodies are critical for reliable data, particularly given the transient expression and low basal levels of FOS proteins. Their application continues to advance understanding of cellular signaling pathways and disease mechanisms.

       
关键字: FOS抗体;FOS;FOS Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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