KBTBD8 (Kelch Repeat and BTB Domain Containing 8) is a protein encoded by the KBTBD8 gene, belonging to the BTB-Kelch superfamily. This family is characterized by the presence of BTB (Broad-Complex, Tramtrack, and Bric-à-brac) domains, which mediate protein-protein interactions, and Kelch repeats, involved in substrate binding. KBTBD8 is thought to function as a substrate-specific adaptor for E3 ubiquitin ligase complexes, potentially regulating protein degradation via the ubiquitin-proteasome system. However, its precise biological roles remain under investigation.
Research suggests KBTBD8 may influence cellular processes such as cell cycle regulation, cytoskeletal organization, and developmental signaling pathways. Notably, studies have linked KBTBD8 to neural crest cell migration during embryogenesis, with dysregulation potentially contributing to neurodevelopmental disorders. Its expression patterns in specific tissues and developmental stages hint at context-dependent functions.
KBTBD8 antibodies are essential tools for detecting and quantifying protein expression, localization, and interactions in experimental models. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study KBTBD8's role in cellular mechanisms and disease contexts. Commercial antibodies are typically validated for specificity through knockdown/knout controls (e.g., siRNA or CRISPR-Cas9) and peptide-blocking assays. Recent studies employing KBTBD8 antibodies have explored its potential involvement in cancer progression and neurological conditions, though further research is needed to fully elucidate its pathophysiological relevance.