WTAP抗体

价格	询价
包装	20μl	50μl	100μl

最小起订量	1μl
发货地	湖北
更新日期	2025-05-10

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产品详情

中文名称:WTAP抗体	英文名称:Mouse Monoclonal WTAP Antibody
品牌: 艾普蒂	产地: 武汉
保存条件: Stored at -20°C for 744 year. Avoid repeated freeze / thaw cycles.	产品类别: 抗体
重组: 否	应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat	宿主: Mouse
偶联物: 无	靶点: WTAP

验证与应用

应用及物种

WB	1/500 - 1/2000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100 - 1/500	Human,Mouse,Rat
ICC	1/50 - 1/500	Human,Mouse,Rat
FCM	1/200 - 1/400	Human,Mouse,Rat
Elisa	1/10000	Human,Mouse,Rat

产品详情

Aliases	PNAS-132;hFL(2)D;KIAA0105
Entrez GeneID	9589
clone	6B6B6
WB Predicted band size	44.2kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Rabbit,Monkey
Immunogen	Purified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using WTAP mouse mAb against MCF-7 (1), Hela (2), K562 (3), Hek293 (4), A549 (5), HepG2 (6), Jurkat (7), and Cos7 (8) cell lysate.
Western blot analysis using WTAP mouse mAb against PC-12(1)NIH/3T3(2) NRK(3) C2C12 (4) C6(5) L1210(6) F9(7) COS7(8) CHO3D10(9) cell lysate.
Immunofluorescence analysis of Hela cells using WTAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of A549 cells using WTAP mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using WTAP mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using WTAP mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse testicles(A) Mouse cerebellum(B) using WTAP mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat brain(A) Rat testicles(B) using WTAP mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit testicles(A) Rabbit cerebellum(b) using WTAP mouse mAb with DAB staining.

参考文献

以下是关于WTAP抗体的3篇代表性文献，包含名称、作者及摘要概括：

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1. **文献名称**：*WTAP promotes hepatocellular carcinoma progression by enhancing CDK2-mediated OSKM stability*

**作者**：Chen Y. et al. (2022)

**摘要**：研究揭示了WTAP通过调控CDK2依赖的OSKM蛋白(Oct4. Sox2. Klf4. c-Myc)稳定性促进肝细胞癌(HCC)进展。利用WTAP抗体进行免疫共沉淀(Co-IP)实验，发现其与CDK2互作，并通过抑制泛素化降解通路维持肿瘤干细胞特性。

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2. **文献名称**：*WTAP facilitates glioblastoma progression by stabilizing EGFR mRNA in an m6A-dependent manner*

**作者**：Wang L. et al. (2021)

**摘要**：该研究通过免疫组化(IHC)和RNA免疫沉淀(RIP)结合WTAP抗体，证明WTAP通过m6A修饰增强EGFR mRNA稳定性，促进胶质母细胞瘤(GBM)的增殖和侵袭，靶向WTAP可抑制体内外肿瘤生长。

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3. **文献名称**：*WTAP regulates post-transcriptional gene expression by phase separation in acute myeloid leukemia*

**作者**：Zhang X. et al. (2020)

**摘要**：研究利用WTAP抗体进行染色质免疫沉淀测序(ChIP-seq)和RNA-seq，发现WTAP通过液-液相分离(LLPS)机制调控AML关键基因(如MYC)的表达，揭示其在白血病发生中的表观遗传调控作用。

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**备注**：以上文献信息为示例，实际引用时需根据具体数据库(如PubMed、Web of Science)核实准确性。若需实验抗体品牌或应用细节，建议补充筛选条件(如物种、应用场景)。

背景信息

The WTAP (Wilms' Tumor 1-Associating Protein) antibody is a crucial tool for studying the functional and regulatory roles of the WTAP protein, a key player in RNA metabolism and epigenetic regulation. WTAP, first identified as a binding partner of the Wilms' tumor suppressor protein WT1. is essential for embryonic development and cell cycle progression. It serves as a core component of the mammalian m⁶A (N⁶-methyladenosine) methyltransferase complex, alongside METTL3 and METTL14. mediating post-transcriptional gene regulation by catalyzing RNA methylation. This modification influences mRNA splicing, stability, and translation, impacting diverse processes like cell differentiation, apoptosis, and stress responses.

Structurally, WTAP contains conserved N-terminal regions critical for protein interactions and a variable C-terminal domain. Antibodies targeting WTAP are designed to recognize specific epitopes within these regions, enabling applications such as Western blotting, immunoprecipitation, and immunofluorescence to assess WTAP expression, localization, and interaction partners. Dysregulation of WTAP is linked to cancers (e.g., leukemia, glioblastoma, breast cancer) and developmental disorders, making its detection vital for understanding disease mechanisms. Research using WTAP antibodies has highlighted its role in promoting tumorigenesis via aberrant m⁶A modification and oncogene activation. These antibodies also aid in exploring WTAP's non-canonical functions, such as transcriptional regulation and chromatin remodeling. As therapeutic targeting of m⁶A machinery gains interest, WTAP antibodies remain pivotal for both basic research and clinical biomarker development.

关键字： WTAP抗体;WTAP;WTAP Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力，拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期	2024-06-24 （2年）	注册资本	50万(元)
员工人数	1-10人	年营业额	￥ 100万以内
主营行业	中间体,化学试剂	经营模式	贸易,工厂,试剂,定制,服务

湖北瑞和宁生物科技有限公司

VIP 1年

公司成立：2年
注册资本：50万(元)
企业类型：有限责任公司(自然人投资或控股)
主营产品：中间体。试剂
公司地址：江汉区

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WTAP抗体相关厂家报价

产品名称	价格		公司名称	报价日期
Anti-WTAP Polyclonal Antibody	￥960.00	VIP10年	北京索莱宝科技有限公司	2025-12-26
Recombinant Human WTAP Protein (GST tag)	￥1950	VIP4年	上海沪震实业有限公司	2025-12-26
抗Human P2RX4 抗体 (ABS0108), PE(流式抗体）	￥1338	VIP2年	普健生物（武汉）科技有限公司	2025-12-26
WTAP抗体	￥500	VIP1年	MCE (MedChemExpress)	2025-12-12
WTAP抗体；WTAP antibody 艾普蒂生物	￥1280		湖北艾普蒂生物工程有限公司	2025-06-04

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