EMD抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of A549 cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Immunofluorescence analysis of Hela cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of K562 cells using EMD mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of A549 cells using EMD mouse mAb (green) and negative control (red).    

  
  

EMD antibodies target the Emerin protein, a key component of the nuclear envelope in eukaryotic cells. Emerin, encoded by the *EMD* gene located on the X chromosome, plays critical roles in maintaining nuclear structure, chromatin organization, and gene regulation. It interacts with lamins (particularly lamin A/C) and other nuclear membrane proteins to stabilize the nuclear lamina, mediate chromatin anchoring, and participate in signaling pathways.

Mutations in *EMD* cause X-linked Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive muscle wasting, joint contractures, and cardiac abnormalities. EMD antibodies are widely used in research and diagnostics to detect Emerin expression levels or localization abnormalities, aiding in EDMD diagnosis and mechanistic studies. These antibodies are employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess nuclear envelope integrity and protein interactions.

Beyond EDMD, Emerin dysfunction is linked to aging-related pathologies and laminopathies, as its interaction with lamin A/C is crucial for genome stability. EMD antibodies also help investigate Emerin's role in stem cell differentiation, DNA repair, and transcriptional regulation. Commercial EMD antibodies are typically raised against specific epitopes, ensuring specificity for both wild-type and mutant variants in clinical or experimental contexts. Their application continues to advance understanding of nuclear envelope biology and its implications in human disease.