GLI1抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of Hela cells using GLI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of HepG2 cells using GLI1 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using GLI1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using GLI1 mouse mAb with DAB staining.    

  
  

The GLI1 antibody is a crucial tool in studying the Hedgehog (Hh) signaling pathway, a key regulator of embryonic development and tissue homeostasis. GLI1. a zinc finger transcription factor, acts as a primary downstream effector of Hh signaling. It mediates the expression of target genes involved in cell proliferation, differentiation, and survival. Unlike GLI2 and GLI3. which can act as both activators and repressors, GLI1 primarily functions as a transcriptional activator. Dysregulation of GLI1 is implicated in various cancers, including basal cell carcinoma, medulloblastoma, and rhabdomyosarcoma, often due to mutations in Hh pathway components (e.g., PTCH1. SMO) or GLI1 amplification.

GLI1 antibodies are widely used in research to detect GLI1 protein expression and localization via techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence. They help assess Hh pathway activation status in tumor samples, stem cell studies, and developmental biology research. Commercially available antibodies target specific epitopes, often within the N-terminal or C-terminal regions, and validation includes checks for specificity using knockout controls. Recent therapeutic strategies targeting GLI1 in cancer have further elevated its research relevance, making these antibodies vital for both mechanistic studies and biomarker development.