The HIST2H4A(20Me3) antibody specifically recognizes histone H4 trimethylated at lysine 20 (H4K20me3), a post-translational modification associated with transcriptional regulation and chromatin organization. Histones, including H4. undergo covalent modifications that influence DNA accessibility and gene expression. H4K20me3 is enriched in constitutive heterochromatin, telomeres, and specific silenced genomic regions, playing roles in maintaining genomic stability, DNA repair, and cell cycle progression. This mark is deposited by the SUV420H1 and SUV420H2 methyltransferases and is linked to the establishment of repressive chromatin states. Dysregulation of H4K20me3 has been implicated in cancer, developmental disorders, and aging-related processes. The HIST2H4A(20Me3) antibody is widely used in epigenetics research to study chromatin dynamics, particularly in models of disease or cellular differentiation. It is validated for applications such as chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting. Proper controls, including peptide competition or knockout cell lines, are essential to confirm specificity due to potential cross-reactivity with similar histone marks. This antibody serves as a critical tool for exploring the functional interplay between histone modifications, chromatin structure, and gene regulation.