PTPN6抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using PTPN6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Raji cells using PTPN6 mouse mAb (green) and negative control (red).    

  
  

PTPN6. also known as SHP-1 (Src Homology 2 domain-containing Phosphatase-1), is a non-receptor protein tyrosine phosphatase encoded by the *PTPN6* gene. It plays a critical role in regulating cellular signaling by dephosphorylating target proteins, thereby modulating immune responses, cell growth, and differentiation. Primarily expressed in hematopoietic cells, SHP-1 acts as a negative regulator of signaling pathways in immune cells, including those mediated by cytokine receptors, antigen receptors, and receptor tyrosine kinases. Its dysfunction has been linked to autoimmune diseases, inflammatory disorders, and malignancies, such as leukemia and lymphoma.

PTPN6 antibodies are essential tools for studying the expression, localization, and function of SHP-1 in both physiological and pathological contexts. These antibodies enable detection of SHP-1 through techniques like Western blotting, immunohistochemistry, and flow cytometry. Researchers use them to investigate SHP-1's role in immune tolerance, tumor suppression, and its paradoxical pro-oncogenic effects in certain cancers. Specific PTPN6 antibodies can distinguish between inactive and active conformational states of the phosphatase, aiding in mechanistic studies of its regulation. Additionally, they are employed in diagnostic and therapeutic research to explore SHP-1-targeted interventions. High specificity and validation are crucial for these antibodies due to structural similarities among phosphatase family members. Understanding SHP-1's dual roles in disease continues to drive interest in developing precise research tools and potential therapies targeting this phosphatase.