AQP2抗体

+ +

•  

   

     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

•  

   

     Western blot analysis using AQP2 mouse mAb against K562 (1), HeLa (2), HCT116 (3), and SW480 (4) cell lysate.    

  
  

Aquaporin-2 (AQP2) antibodies are essential tools for studying the regulation of water homeostasis in the kidney. AQP2 is a water channel protein primarily expressed in the principal cells of the renal collecting duct, where it mediates water reabsorption under the control of antidiuretic hormone (vasopressin). Its function is critical for maintaining body fluid balance, and dysregulation of AQP2 is linked to disorders like nephrogenic diabetes insipidus (NDI), heart failure, and hypertension.

AQP2 antibodies are widely used in research to detect AQP2 expression, localization, and trafficking in both physiological and pathological contexts. For example, they help investigate how vasopressin signaling (via V2 receptors) triggers AQP2 phosphorylation and translocation to the apical membrane, enhancing water permeability. These antibodies are employed in techniques such as immunohistochemistry, Western blotting, and immunofluorescence to analyze tissue or cellular AQP2 distribution.

Clinically, AQP2 antibodies aid in diagnosing AQP2-related disorders. Mutations in the AQP2 gene or signaling defects can reduce its membrane expression, leading to polyuria and polydipsia in NDI. Additionally, urinary exosome-derived AQP2 quantification using antibodies serves as a non-invasive biomarker for renal water handling. Commercial AQP2 antibodies are typically raised in rabbits or mice against specific epitopes (e.g., C-terminal or N-terminal regions), with validation in knockout models ensuring specificity. Their applications extend to studying drug mechanisms (e.g., vasopressin analogs) and systemic diseases affecting renal function.