Dynamin-2 (DNM2) is a large GTPase belonging to the dynamin superfamily, widely expressed in eukaryotic cells and essential for membrane fission and remodeling processes. It plays a critical role in clathrin-mediated endocytosis, intracellular vesicle trafficking, and organelle maintenance, including mitochondrial division. Unlike dynamin-1. which is neuron-specific, DNM2 is ubiquitously expressed and regulates diverse cellular functions such as cytoskeletal dynamics, cell migration, and receptor signaling. Mutations in the *DNM2* gene are linked to human diseases, including centronuclear myopathy (CNM) and Charcot-Marie-Tooth neuropathy (CMT), highlighting its physiological importance.
DNM2 antibodies are immunological tools designed to detect and study the expression, localization, and functional interactions of dynamin-2 in research. These antibodies, often developed in rabbit or mouse hosts, target specific epitopes within DNM2 domains (e.g., GTPase, pleckstrin homology, or proline-rich domains). They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate DNM2’s role in cellular processes or disease mechanisms. Specific DNM2 antibodies can distinguish between isoforms or post-translationally modified forms, aiding studies on phosphorylation or disease-associated mutations. Validation across species (human, mouse, rat) ensures versatility in experimental models. Research applications range from analyzing membrane trafficking defects in genetic disorders to exploring DNM2’s involvement in cancer cell invasiveness or viral entry pathways. Proper antibody selection and validation are critical, as DNM2 shares structural homology with other dynamin family members.