RCHY1抗体,Rabbit Polyclonal RCHY1 Antibody
  • RCHY1抗体,Rabbit Polyclonal RCHY1 Antibody
  • RCHY1抗体,Rabbit Polyclonal RCHY1 Antibody
  • RCHY1抗体,Rabbit Polyclonal RCHY1 Antibody

RCHY1抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-11
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产品详情

中文名称:RCHY1抗体英文名称:Rabbit Polyclonal RCHY1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 1738 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: RCHY1
2025-05-11 RCHY1抗体 Rabbit Polyclonal RCHY1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 1738 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/200 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/5000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996
WB Predicted band size30 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFusion protein of human RCHY1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-4: 293T, A549, Jurkat, 231 cell lysates, Primary antibody: P00627(RCHY1 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 30 seconds    


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       The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P00627(RCHY1 Antibody) at dilution 1/50. (Original magnification: ×200)    


           

参考文献

以下是关于RCHY1抗体的模拟参考文献示例(文献为假设性内容,仅供格式参考):

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1. **文献名称**: "RCHY1 promotes p53 ubiquitination and degradation to modulate tumor progression"

**作者**: Shmueli, A. et al.

**摘要**: 该研究揭示了RCHY1(PIRH2)通过泛素-蛋白酶体途径促进p53蛋白降解的机制,利用特异性抗体证实其在癌细胞中与p53的相互作用,为肿瘤发生提供了新的分子机制解释。

2. **文献名称**: "High expression of RCHY1 correlates with poor prognosis in hepatocellular carcinoma"

**作者**: Zhang, L. et al.

**摘要**: 通过免疫组化(使用RCHY1抗体)分析肝癌患者组织样本,发现RCHY1高表达与患者生存期缩短显著相关,并证实其通过激活AKT通路促进肿瘤侵袭。

3. **文献名称**: "MicroRNA-34a suppresses RCHY1 expression to inhibit lung cancer cell proliferation"

**作者**: Li, Y. et al.

**摘要**: 研究报道miR-34a通过靶向抑制RCHY1的翻译(Western blot验证使用RCHY1抗体),进而阻滞肺癌细胞周期进程,提示其作为潜在治疗靶点。

4. **文献名称**: "Development of a monoclonal antibody against RCHY1 for therapeutic targeting in breast cancer"

**作者**: Wang, Q. et al.

**摘要**: 该团队开发了一种高特异性抗RCHY1单克隆抗体,体外实验显示其能有效抑制乳腺癌细胞迁移,动物模型中联合化疗药物显著降低肿瘤负荷。

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**注**:以上文献为示例性内容,实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。

       

背景信息

The RCHY1 antibody targets the RCHY1 protein, also known as PIRH2 (p53-induced RING-H2 protein), a member of the RING finger family of E3 ubiquitin ligases. RCHY1 plays a critical role in regulating protein degradation via the ubiquitin-proteasome system, particularly in cell cycle control, apoptosis, and DNA damage response. It interacts with and ubiquitinates several key substrates, including the tumor suppressor p53. promoting its proteasomal degradation, thereby linking RCHY1 to oncogenic processes. Overexpression of RCHY1 has been observed in various cancers, correlating with poor prognosis due to reduced p53 activity.

Structurally, RCHY1 contains a C-terminal RING domain essential for its E3 ligase activity and an N-terminal nuclear localization signal. Its expression is transcriptionally induced by p53. forming a negative feedback loop to modulate p53 levels post-stress. Dysregulation of this pathway is implicated in tumorigenesis and chemoresistance.

RCHY1 antibodies are widely used in research to study protein interactions, expression patterns, and subcellular localization in cancer models. They are critical tools for Western blotting, immunoprecipitation, and immunohistochemistry, aiding in the exploration of RCHY1's role in cellular homeostasis and disease. Recent studies also investigate RCHY1 inhibitors as potential therapeutic agents to restore p53 function in cancers.

       
关键字: RCHY1抗体;RCHY1;RCHY1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (1年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:1年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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