WDR83 (WD repeat domain 83) is a protein encoded by the WDR83 gene, belonging to the WD40 repeat protein family. These proteins are characterized by conserved repeats of ~40 amino acids, typically forming β-propeller structures that mediate protein-protein interactions. WDR83 plays a role in cellular processes such as signal transduction, transcriptional regulation, and macromolecular complex assembly. It interacts with various partners, including the mammalian target of rapamycin (mTOR) pathway components, suggesting involvement in nutrient sensing and cell growth regulation. Studies also link WDR83 to the ERK signaling cascade and retrograde transport from endosomes to the Golgi apparatus.
Antibodies targeting WDR83 are essential tools for investigating its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study WDR83's role in physiological and pathological contexts. For instance, WDR83 has been implicated in cancer progression, metabolic disorders, and viral infections, making its antibodies valuable for exploring disease mechanisms. Researchers often validate these antibodies using knockout cell lines or siRNA-mediated knockdown to ensure specificity. Commercial WDR83 antibodies are typically developed in hosts like rabbits or mice, with options for monoclonal or polyclonal formats. Proper antibody characterization is critical, as off-target binding can occur due to structural similarities among WD40-repeat proteins.