The TMSB4X antibody targets thymosin β4 (Tβ4), a small, highly conserved protein encoded by the TMSB4X gene. Tβ4 binds to actin, regulating cytoskeletal dynamics by sequestering monomeric actin (G-actin) and promoting polymerization into filamentous actin (F-actin). This interaction influences cell motility, tissue repair, inflammation, and angiogenesis. TMSB4X is ubiquitously expressed, with high levels in blood cells, endothelial cells, and certain tumor cells. Antibodies against Tβ4 are essential tools for studying its roles in physiological and pathological processes, such as wound healing, cardiovascular disease, cancer metastasis, and autoimmune disorders. In research, TMSB4X antibodies enable detection of protein expression via Western blot, immunohistochemistry, or immunofluorescence, helping elucidate its localization and interaction networks. Dysregulation of Tβ4 has been linked to tumor progression (e.g., promoting invasion in cancers) and inflammatory conditions, making its antibody valuable for diagnostic or therapeutic exploration. However, functional redundancy within the β-thymosin family and post-translational modifications may complicate antibody specificity, necessitating rigorous validation. Overall, TMSB4X antibodies contribute to understanding Tβ4's dual roles as a cytoskeletal modulator and signaling molecule in health and disease.