The heterogeneous nuclear ribonucleoprotein M (HNRNPM) is a member of the hnRNP family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, transport, stability, and translation. HNRNPM contains RNA recognition motifs (RRMs) that enable binding to specific RNA sequences, influencing alternative splicing decisions and mRNA processing. It is implicated in regulating genes involved in cell proliferation, DNA repair, and stress responses.
HNRNPM antibodies are essential tools in molecular biology research, used to detect and study the protein's expression, localization, and interactions in various cellular contexts. These antibodies are widely applied in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Dysregulation of HNRNPM has been linked to cancers (e.g., breast cancer, glioblastoma) and neurological disorders, making its study clinically relevant. For instance, HNRNPM overexpression in tumors is associated with enhanced oncogenic splicing events and metastasis.
Research using HNRNPM antibodies has helped uncover its role in epitranscriptomic regulation, including interactions with m6A-modified RNAs. Such studies highlight its potential as a therapeutic target. Validated antibodies with high specificity are crucial to avoid cross-reactivity with other hnRNP family members, ensuring accurate experimental outcomes in both basic and translational research.