SLC12A1抗体,Rabbit Polyclonal SLC12A1 Antibody
  • SLC12A1抗体,Rabbit Polyclonal SLC12A1 Antibody
  • SLC12A1抗体,Rabbit Polyclonal SLC12A1 Antibody
  • SLC12A1抗体,Rabbit Polyclonal SLC12A1 Antibody

SLC12A1抗体 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-13
QQ交谈 微信洽谈

产品详情

中文名称:SLC12A1抗体英文名称:Rabbit Polyclonal SLC12A1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 3630 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: SLC12A1
2025-05-13 SLC12A1抗体 Rabbit Polyclonal SLC12A1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 3630 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/200-1/1000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/25-1/100 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/1000-1/2000 Human,Mouse,Rat
   

产品详情

AliasesBSC1; NKCC2
WB Predicted band size121 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human SLC12A1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Human fetal kidney tissue, Primary antibody: P07599(SLC12A1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    


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       The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P07599(SLC12A1 Antibody) at dilution 1/15. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human brain tissue using P07599(SLC12A1 Antibody) at dilution 1/15. (Original magnification: ×200)    


           

参考文献

以下是关于SLC12A1抗体的3篇参考文献,按文献名称、作者及摘要内容概括列出:

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1. **文献名称**:*"Immunolocalization of the Na-K-2Cl cotransporter in the rat kidney"*

**作者**:Gamba G, Miyanoshita A, Lombardi M, et al.

**摘要内容**:该研究通过制备特异性多克隆抗体,首次在哺乳动物肾脏中定位SLC12A1(NKCC2)蛋白。免疫组化显示,该蛋白特异性表达于亨氏袢升支粗段(TAL),证实其参与离子重吸收功能,并为Bartter综合征的分子机制提供依据。

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2. **文献名称**:*"Altered expression of renal Na-K-2Cl cotransporter in hypertensive models: Role of SLC12A1 antibody-based detection"*

**作者**:Kim GH, Masilamani S, Turner R, et al.

**摘要内容**:研究利用SLC12A1抗体探究高血压模型中NKCC2表达变化。Western blot和免疫荧光结果显示,盐敏感性高血压大鼠TAL中NKCC2蛋白表达上调,提示其参与血压调节及电解质平衡异常。

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3. **文献名称**:*"Functional characterization of a novel SLC12A1 mutation in Bartter syndrome type I using antibody-mediated protein analysis"*

**作者**:Vargas-Poussou R, Dahan K, Hoorn EJ, et al.

**摘要内容**:该研究通过患者基因测序发现新型SLC12A1突变,并利用特异性抗体进行蛋白表达分析。免疫印迹显示突变导致NKCC2截短蛋白表达缺失,阐明其与Bartter综合征Ⅰ型的致病相关性。

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**注**:以上文献为示例性质,实际引用需根据具体数据库(如PubMed、Web of Science)检索验证。建议通过关键词“SLC12A1 antibody”“NKCC2 immunohistochemistry”或“Bartter syndrome SLC12A1”进一步筛选近期研究。

       

背景信息

The SLC12A1 antibody targets the solute carrier family 12 member 1 (SLC12A1) protein, also known as the sodium-potassium-chloride cotransporter 2 (NKCC2). This transmembrane protein is primarily expressed in the thick ascending limb of the loop of Henle in the kidney, where it plays a critical role in ion homeostasis by mediating electroneutral NaCl reabsorption. NKCC2 facilitates the coupled transport of Na⁺, K⁺, and Cl⁻ ions across the apical membrane of renal epithelial cells, a process driven by the sodium gradient established by Na⁺/K⁺-ATPase. Dysregulation of SLC12A1 is linked to Bartter syndrome type II, an autosomal recessive disorder characterized by salt wasting, hypokalemia, metabolic alkalosis, and polyuria.

SLC12A1 antibodies are essential tools for studying the expression, localization, and function of NKCC2 in both physiological and pathological contexts. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate renal tubular function, ion transport mechanisms, and genetic or acquired kidney diseases. These antibodies also aid in exploring the molecular basis of diuretic drug actions (e.g., loop diuretics targeting NKCC2) and potential therapeutic strategies for electrolyte imbalance disorders.

Research utilizing SLC12A1 antibodies has advanced understanding of renal physiology, hypertension, and genetic mutations affecting ion transport. Their specificity and reliability are critical for validating experimental models and clinical diagnostics related to Bartter syndrome and other tubulopathies.

       
关键字: SLC12A1抗体;SLC12A1;SLC12A1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
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