DIABLO抗体,Rabbit Polyclonal DIABLO Antibody
  • DIABLO抗体,Rabbit Polyclonal DIABLO Antibody
  • DIABLO抗体,Rabbit Polyclonal DIABLO Antibody
  • DIABLO抗体,Rabbit Polyclonal DIABLO Antibody

DIABLO抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-13
QQ交谈 微信洽谈

产品详情

中文名称:DIABLO抗体英文名称:Rabbit Polyclonal DIABLO Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 3633 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: DIABLO
2025-05-13 DIABLO抗体 Rabbit Polyclonal DIABLO Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 3633 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/1000-1/5000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/200 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/2000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesGLBA; SAP1
Entrez GeneID5660
clone4D5F4
WB Predicted band size58.1kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human PSAP (AA: 325-524 ) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Immunofluorescence analysis of HepG2 cells using PSAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of HeLa cells using PSAP mouse mAb (green) and negative control (purple).    


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       mmunohistochemical analysis of paraffin-embedded human pancreas tissues using PSAP mouse mAb with DAB staining.    


           

参考文献

以下是关于DIABLO(SMAC)抗体的3篇经典文献,涵盖其功能研究与抗体应用:

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1. **文献名称**:*Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins*

**作者**:Verhagen, A.M., et al.

**摘要**:该研究首次鉴定了DIABLO蛋白,通过生成特异性多克隆抗体,证实其在细胞凋亡过程中从线粒体释放,并与凋亡抑制蛋白(IAP)结合,解除IAP对caspase的抑制作用,从而促进细胞凋亡。

2. **文献名称**:*Smac, a mitochondrial protein that promotes cytochrome c–dependent caspase activation by eliminating IAP inhibition*

**作者**:Du, C., et al.

**摘要**:本研究独立发现了SMAC(即DIABLO),利用抗体通过免疫印迹和免疫荧光技术,证明其通过拮抗IAP(如XIAP)促进caspase-9激活,并揭示了其在凋亡信号通路中的关键作用。

3. **文献名称**:*Targeting of DIABLO/SMAC for therapeutic intervention in cancer*

**作者**:Yang, Q.H., et al.

**摘要**:通过抗DIABLO抗体分析其在肿瘤组织中的表达,研究发现DIABLO在多种癌症中表达异常,并探讨了通过调控DIABLO-IAP相互作用增强化疗敏感性的潜在治疗策略。

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以上文献均涉及DIABLO抗体的应用,包括功能验证(如Western blot、免疫荧光)及临床相关性研究,为凋亡机制及癌症治疗提供了重要依据。如需更近期研究或特定抗体开发文献,建议进一步限定时间范围或应用场景。

       

背景信息

The DIABLO (Direct IAP-Binding Protein with Low pI) protein, also known as Smac (Second mitochondria-derived activator of caspases), is a mitochondrial pro-apoptotic factor critical for regulating programmed cell death. It is released into the cytosol during apoptosis, where it neutralizes inhibitor of apoptosis proteins (IAPs) by binding to them, thereby relieving their suppression of caspase activity. This interaction promotes caspase activation and execution of apoptosis.

Antibodies targeting DIABLO/Smac are essential tools for studying its expression, localization, and function in cellular pathways. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess DIABLO levels in cancer research, as dysregulation of DIABLO is linked to tumor progression and chemoresistance. Reduced DIABLO expression in some cancers correlates with evasion of apoptosis, making it a potential biomarker or therapeutic target.

Additionally, DIABLO antibodies aid in exploring its role in neurodegenerative diseases and ischemic injuries, where impaired apoptosis regulation contributes to pathology. The development of SMAC mimetics—small molecules mimicking DIABLO’s IAP-binding function—highlights its therapeutic relevance. These mimetics, combined with antibody-based studies, advance understanding of apoptosis mechanisms and strategies to restore cell death sensitivity in treatment-resistant diseases. Research on DIABLO continues to uncover its broader roles in cellular stress responses and immune regulation.

       
关键字: DIABLO抗体;DIABLO;DIABLO Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
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