**Background of MYLK3 Antibody**
MYLK3 (myosin light chain kinase 3) is a member of the myosin light chain kinase family, which regulates smooth muscle and non-muscle cell contraction by phosphorylating myosin regulatory light chains. Unlike other MYLK isoforms (e.g., MYLK1/MYLK2), MYLK3 is predominantly expressed in cardiac and skeletal muscle tissues, where it plays a role in modulating muscle function and stress responses. Studies suggest MYLK3 is involved in cardiac development, contractility, and adaptation to mechanical or pathological stimuli, though its precise mechanisms remain less characterized compared to other family members.
The MYLK3 antibody is a critical tool for detecting and quantifying MYLK3 protein expression in research. It enables investigations into MYLK3's subcellular localization, interaction partners, and phosphorylation dynamics, particularly in cardiac hypertrophy, heart failure, or muscular disorders. Commercial MYLK3 antibodies are typically developed in rabbits or mice using immunogenic peptide sequences unique to MYLK3. ensuring specificity. Validation often includes Western blotting, immunohistochemistry, or immunofluorescence in relevant tissues or cell lines.
Recent interest in MYLK3 stems from its potential role in cardiomyopathies and as a biomarker for cardiac dysfunction. However, functional studies remain limited, underscoring the need for reliable antibodies to advance understanding of MYLK3's physiological and pathological contributions.