The KIF25 antibody is a research tool designed to detect and study kinesin family member 25 (KIF25), a protein belonging to the kinesin superfamily. Kinesins are microtubule-dependent motor proteins involved in intracellular transport, mitosis, and cell signaling. KIF25. specifically, is a relatively less characterized member of this family. It contains a conserved kinesin motor domain at its N-terminus, enabling ATP-dependent movement along microtubules, and a divergent C-terminal region that may mediate interactions with cargo molecules or regulatory partners.
KIF25 is implicated in cellular processes such as organelle trafficking, spindle dynamics during cell division, and possibly cancer progression. Studies suggest its overexpression in certain cancers, including colorectal and breast cancer, may correlate with tumor aggressiveness. The KIF25 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate its expression, localization, and functional roles in both physiological and pathological contexts.
Validation of KIF25 antibodies typically involves testing specificity via knockout cell lines or siRNA-mediated knockdown. Researchers rely on these antibodies to explore KIF25's contributions to cellular dynamics and disease mechanisms, though its precise biological functions and regulatory pathways remain under active investigation.