The GPN2 antibody is a research tool designed to target the GPN-loop GTPase 2 protein, a conserved eukaryotic enzyme encoded by the *GPN2* gene. GPN2 belongs to the GPN (GLYCOPIN) family of GTPases, which play critical roles in RNA polymerase II (RNAP II) assembly, nuclear transport, and DNA repair. Structurally, GPN2 contains a distinctive glycine-proline-asparagine (GPN) motif, essential for its GTPase activity and interaction with RNAP II subunits. Studies suggest that GPN2 collaborates with GPN1 and GPN3 to form a trimeric complex that stabilizes RNAP II during its biogenesis and nuclear localization. Dysregulation of GPN2 has been linked to transcriptional defects, genomic instability, and pathologies such as cancer, where its overexpression correlates with tumor progression.
The GPN2 antibody is widely used in molecular biology to investigate protein expression, localization, and function through techniques like Western blotting, immunofluorescence, and immunoprecipitation. It aids in elucidating mechanisms of RNAP II dynamics, cell cycle regulation, and stress responses. Commercial GPN2 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins as immunogens, and their specificity is validated via knockout controls or siRNA-mediated knockdown. As RNAP II dysfunction is implicated in numerous diseases, this antibody remains vital for advancing studies in transcription biology, cancer research, and therapeutic target discovery.