ORC1抗体

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P09801(ORC1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

ORC1 (Origin Recognition Complex subunit 1) antibodies are essential tools for studying the molecular mechanisms of DNA replication initiation in eukaryotic cells. The ORC1 protein is a critical component of the Origin Recognition Complex (ORC), a six-subunit complex (ORC1-6) that binds to replication origins to initiate DNA synthesis during the cell cycle. ORC1 plays a central role in recruiting additional replication factors, such as CDC6 and the MCM helicase complex, ensuring proper licensing of replication origins during the G1 phase. Dysregulation of ORC1 function is linked to replication errors, genomic instability, and diseases like Meier-Gorlin syndrome, a primordial dwarfism disorder associated with mutations in ORC1.

ORC1 antibodies are widely used in research to investigate cell cycle regulation, DNA replication dynamics, and cancer biology. These antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions) of human ORC1. They enable techniques such as Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and flow cytometry to detect ORC1 expression, localization, and interactions. Commercial ORC1 antibodies are often validated for specificity using knockout cell lines or siRNA knockdowns. Researchers also utilize them to explore ORC1's role in development, stem cell biology, and diseases marked by replication stress, providing insights into therapeutic targets for cancer and genetic disorders.