The RhCG antibody is a crucial tool in biomedical research for studying the Rh family C glycoprotein (RhCG), a member of the Rh-associated glycoprotein family. RhCG, encoded by the *RHCG* gene, is primarily expressed in the kidneys, particularly in renal tubular epithelial cells, where it plays a vital role in ammonium transport and systemic acid-base homeostasis. Unlike RhD and RhCE antigens found on erythrocytes, RhCG is a non-erythroid protein involved in facilitating ammonia secretion, critical for maintaining pH balance by excreting excess acid. Research on RhCG has implications for understanding renal physiology, tubular acidosis, and disorders linked to ammonia metabolism. The RhCG antibody enables the detection and localization of RhCG in tissues through techniques like immunohistochemistry, Western blotting, and immunofluorescence. It has been instrumental in elucidating RhCG’s distribution in the distal nephron, including intercalated cells of the collecting duct, and its interaction with other ammonium transporters. Studies using RhCG antibodies have also explored its role in adaptive responses to metabolic acidosis and chronic kidney disease. Commercial RhCG antibodies are typically raised in rabbits or mice, targeting specific epitopes of the protein. Validation includes testing in knockout models to confirm specificity. Overall, RhCG antibodies are essential for advancing research into renal ammonia handling and related pathologies.