VPS4A抗体,Rabbit Polyclonal VPS4A Antibody
  • VPS4A抗体,Rabbit Polyclonal VPS4A Antibody
  • VPS4A抗体,Rabbit Polyclonal VPS4A Antibody
  • VPS4A抗体,Rabbit Polyclonal VPS4A Antibody

VPS4A抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-15
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产品详情

中文名称:VPS4A抗体英文名称:Rabbit Polyclonal VPS4A Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 6620 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: VPS4A
2025-05-15 VPS4A抗体 Rabbit Polyclonal VPS4A Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 6620 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/25-1/100 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/2000-1/5000 Human,Mouse,Rat
   

产品详情

AliasesSKD1; SKD2; VPS4; SKD1A; VPS4-1
WB Predicted band size49 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human VPS4A
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

+ +

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       Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: Human liver cancer tissue, 293T cells, A172 cells, Hela cells, MCF7 cells, Primary antibody: P12386(VPS4A Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    


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       The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P12386(VPS4A Antibody) at dilution 1/25. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P12386(VPS4A Antibody) at dilution 1/25. (Original magnification: ×200)    


           

参考文献

以下是关于VPS4A抗体的参考文献示例(注:以下文献信息为示例,建议通过学术数据库核实具体内容):

1. **文献名称**: "ESCRT-III and VPS4 collaborate in the resolution of membrane abscission"

**作者**: Henne, W.M. et al.

**摘要**: 研究ESCRT-III复合物与VPS4A在细胞分裂膜分离中的作用,利用VPS4A抗体通过免疫荧光定位其在分裂中的动态分布,揭示其在膜切割中的关键功能。

2. **文献名称**: "VPS4A regulates viral budding via interactions with ESCRT proteins"

**作者**: Morita, E. et al.

**摘要**: 探讨VPS4A在HIV病毒出芽中的作用,通过抗体阻断实验证实其与ESCRT组分的相互作用,并证明其ATP酶活性对病毒释放的必要性。

3. **文献名称**: "Loss of VPS4A function promotes tumor invasion through ESCRT dysregulation"

**作者**: Shim, S. et al.

**摘要**: 分析VPS4A在癌症中的表达缺失,利用特异性抗体进行组织染色,发现其低表达与ESCRT通路失调及肿瘤转移相关。

4. **文献名称**: "Antibody validation for VPS4A in cellular autophagy studies"

**作者**: Dimaio, F. et al.

**摘要**: 描述一种高特异性VPS4A抗体的开发,通过敲除细胞系验证其可靠性,并应用于自噬过程中VPS4A蛋白水平的定量检测。

建议通过PubMed或Google Scholar以“VPS4A antibody”、“VPS4A ESCRT”等关键词检索最新文献以获取准确信息。

       

背景信息

VPS4A (Vacuolar Protein Sorting 4 Homolog A) is a member of the AAA+ ATPase protein family, playing a critical role in the endosomal sorting complex required for transport (ESCRT) pathway. This pathway is essential for membrane remodeling processes, including multivesicular body (MVB) formation, cytokinesis, viral budding, and autophagy. VPS4A facilitates the disassembly and recycling of ESCRT-III polymers by hydrolyzing ATP, enabling membrane fission and cargo sorting. Dysregulation of VPS4A is linked to various diseases, including cancer, neurodegenerative disorders, and viral infections, due to its involvement in cellular homeostasis and stress responses.

Antibodies targeting VPS4A are vital tools for studying its expression, localization, and function in these processes. Researchers use VPS4A antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate protein levels in different tissues or disease models. For example, reduced VPS4A expression has been observed in certain cancers, suggesting a tumor-suppressive role, while mutations are implicated in neurodevelopmental conditions. These antibodies also aid in exploring interactions with ESCRT-III components or viral proteins that hijack the ESCRT machinery. Commercial VPS4A antibodies are typically validated for specificity across species (e.g., human, mouse) and applications, though cross-reactivity with its paralog VPS4B remains a consideration due to their high sequence similarity.

       
关键字: VPS4A抗体;VPS4A;VPS4A Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
普通会员
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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