The RBFOX2 antibody is a crucial tool for studying the RBFOX2 protein, a member of the RNA-binding Fox (Rbfox) family. RBFOX2. also known as RBM9 or FOX2. is a conserved RNA-binding protein that regulates alternative splicing, polyadenylation, and mRNA stability by binding to UGCAUG motifs in target RNAs. It plays a vital role in diverse cellular processes, including neuronal development, muscle differentiation, cell cycle regulation, and cell migration. Dysregulation of RBFOX2 is linked to cancers, neurodevelopmental disorders, and cardiovascular diseases.
RBFOX2 antibodies are widely used in research applications such as Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and interactions. These antibodies help elucidate RBFOX2’s tissue-specific functions, particularly in the brain, heart, and muscles. Commercial RBFOX2 antibodies are typically raised against specific epitopes (e.g., N- or C-terminal regions) and validated for specificity across human, mouse, and rat models. Recent studies also explore its role in cancer progression and metastasis, highlighting its potential as a therapeutic target. Proper validation, including knockout controls, is essential to ensure antibody reliability due to possible cross-reactivity with homologous proteins like RBFOX1/3.