alphaTubulin（yeast）抗体—艾普蒂 新品

+ +

•  

   

     Western blot analysis of alpha Tubulin expression in Saccharomyces cerevisiae (yeast) cell lysate.    

  
  

AlphaTubulin is a highly conserved structural protein essential for forming microtubules, dynamic cytoskeletal components critical for various cellular processes including chromosome segregation during mitosis, intracellular transport, and maintenance of cell shape. In yeast, alpha-tubulin (encoded by the TUB1 and TUB3 genes in budding yeast, or the atb2+ gene in fission yeast) pairs with beta-tubulin to form heterodimers, the building blocks of microtubules. Due to its conserved nature and functional significance, alpha-tubulin serves as a widely used loading control in Western blotting and a marker for microtubule visualization in immunofluorescence studies.

Antibodies targeting yeast alpha-tubulin are specifically developed to recognize epitopes unique to fungal tubulin isoforms, minimizing cross-reactivity with mammalian homologs. These antibodies are crucial tools in studying yeast cell biology, particularly in experiments exploring cell cycle regulation, microtubule dynamics, or responses to anti-microtubule drugs (e.g., benomyl). They enable researchers to monitor tubulin expression levels, post-translational modifications (e.g., acetylation, tyrosination), and microtubule organization under varying genetic or environmental conditions. Both monoclonal and polyclonal versions are available, often validated for applications like immunoblotting, immunoprecipitation, and fluorescence microscopy in Saccharomyces cerevisiae or Schizosaccharomyces pombe models. Their reliability in quantitative assays makes them indispensable for normalizing protein samples and ensuring experimental reproducibility in yeast studies.