The TONSL antibody targets the TONSL protein (TonSL homolog), a key player in maintaining genome stability and chromatin replication. TONSL interacts with the histone H4K20me2 mark and the replication stress response protein SMARCAL1. facilitating replication fork restart and repair of DNA lesions during S phase. It is critical for the replication stress response, ensuring proper chromatin restoration after DNA replication. Dysregulation of TONSL is linked to genomic instability, a hallmark of cancers and developmental disorders.
Antibodies against TONSL are widely used in research to study DNA repair mechanisms, replication stress pathways, and chromatin remodeling. They enable detection of TONSL expression levels, localization studies (e.g., via immunofluorescence), and functional assays (e.g., co-immunoprecipitation to identify interacting partners). Studies using TONSL antibodies have revealed its role in recruiting SMARCAL1 to stalled forks (Räschle et al., 2015) and its overexpression in certain cancers, correlating with poor prognosis. Recent work also highlights TONSL as a potential therapeutic target due to its involvement in chemotherapy resistance. These antibodies thus serve as essential tools for unraveling replication-associated pathologies and developing targeted therapies.