RUVBL2 (RuvB-like AAA ATPase 2) is a conserved eukaryotic protein belonging to the AAA+ (ATPases Associated with diverse cellular Activities) superfamily. It forms a heterohexameric complex with RUVBL1. functioning as a critical ATP-dependent molecular chaperone in multiple cellular processes, including chromatin remodeling, transcriptional regulation, DNA repair, and telomerase assembly. The RUVBL1/2 complex interacts with key complexes like INO80. SWR1. and TIP60 to modulate histone exchange, nucleosome positioning, and DNA damage response. Dysregulation of RUVBL2 is linked to cancer, neurodegenerative disorders, and developmental defects, highlighting its role in cell proliferation and genomic stability.
Antibodies targeting RUVBL2 are essential tools for investigating its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study tissue-specific expression patterns, subcellular distribution (primarily nuclear), and dynamic roles in oncogenic pathways. Commercial RUVBL2 antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown to minimize cross-reactivity. Researchers also employ these antibodies to explore RUVBL2's involvement in cancer progression, stem cell maintenance, and c-MYC-driven transcriptional programs, making them valuable for both basic research and therapeutic target validation.