The SPIRE1 antibody is a research tool designed to target the SPIRE1 protein, a member of the SPIRE family of actin nucleation-promoting factors. SPIRE1 (encoded by the *SPIRE1* gene in humans) plays a role in regulating actin cytoskeleton dynamics, essential for cellular processes like vesicle trafficking, cell motility, and intracellular transport. It collaborates with formin proteins to nucleate and elongate actin filaments, influencing cell shape, polarity, and organelle positioning. SPIRE1 is particularly noted for its involvement in oocyte maturation and early embryonic development, as well as in specialized secretory pathways.
The antibody is commonly used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to detect SPIRE1 expression, localization, and interactions in cells or tissues. Researchers employ it to study SPIRE1's functional roles in physiological contexts (e.g., neuronal development, reproductive biology) and pathological conditions, including cancer metastasis or infertility, where SPIRE1 dysregulation has been implicated. Validation of antibody specificity is critical, often confirmed via knockout controls or siRNA-based silencing. As actin dynamics are central to many diseases, SPIRE1 antibodies contribute to understanding molecular mechanisms and potential therapeutic targets. Commercial SPIRE1 antibodies are typically raised in hosts like rabbits or mice, with availability varying by supplier and application validation.