The TLE6 antibody targets the Transducin-like enhancer of split 6 (TLE6) protein, a member of the Groucho/TLE family of transcriptional corepressors. These proteins lack DNA-binding domains but interact with transcription factors to modulate gene expression, primarily through chromatin remodeling and recruitment of histone deacetylases. TLE6 is distinct from other TLE family members due to its specific role in embryonic development, particularly in oocytes and early embryos. It is critical for cell polarity, spindle assembly, and asymmetric cell division during mammalian preimplantation development.
TLE6 forms a subcortical maternal complex (SCM) with other proteins like MATER and FILIA, essential for zygotic genome activation and embryogenesis. Mutations in TLE6 are linked to female infertility and early embryonic arrest in humans. Research on TLE6 antibodies focuses on elucidating its expression patterns, localization, and interactions in developmental models. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study protein dynamics in reproductive biology and stem cell research.
Commercial TLE6 antibodies are typically monoclonal or polyclonal, validated for specificity in human, mouse, or rat samples. Their applications extend to investigating molecular mechanisms underlying developmental disorders and improving assisted reproductive technologies.