The USP43 antibody is a tool used to study ubiquitin-specific protease 43 (USP43), a member of the deubiquitinating enzyme (DUB) family. USP43 belongs to the ubiquitin-specific protease (USP) subclass, which cleaves ubiquitin from protein substrates to regulate their stability, localization, or activity. Ubiquitination is a key post-translational modification involved in diverse cellular processes, including protein degradation, DNA repair, and signal transduction. By reversing this process, USP43 is hypothesized to modulate pathways linked to cancer, metabolism, or epigenetic regulation, though its precise biological roles remain under investigation.
USP43 antibodies are typically developed to detect endogenous USP43 protein in experimental settings, such as Western blotting, immunofluorescence, or immunoprecipitation. They enable researchers to explore USP43 expression patterns across tissues, its subcellular localization, and interactions with other molecules. Some studies suggest USP43 may influence histone modification (e.g., H2BK120 ubiquitination) or participate in pathways like Wnt/β-catenin signaling, but mechanistic details are still emerging. Aberrant USP43 expression has been tentatively associated with diseases like colorectal cancer, making it a potential therapeutic target. However, the antibody's utility depends on its specificity and validation in relevant models, as cross-reactivity with other USPs remains a challenge. Ongoing research aims to clarify USP43's physiological and pathological significance using such reagents.