BUB3抗体,Rabbit Polyclonal BUB3 Antibody
  • BUB3抗体,Rabbit Polyclonal BUB3 Antibody
  • BUB3抗体,Rabbit Polyclonal BUB3 Antibody
  • BUB3抗体,Rabbit Polyclonal BUB3 Antibody

BUB3抗体—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-19
QQ交谈 微信洽谈

产品详情

中文名称:BUB3抗体英文名称:Rabbit Polyclonal BUB3 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 4174 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: BUB3
2025-05-19 BUB3抗体 Rabbit Polyclonal BUB3 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 4174 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/2000-1/5000 Human,Mouse,Rat
   

产品详情

AliasesBUB3L; hBUB3
WB Predicted band size37 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFusion protein of human BUB3
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Jurkat cells, Hela cells, Primary antibody: P08494(BUB3 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    


           

参考文献

以下是关于BUB3抗体的3篇参考文献示例(文献信息为模拟内容,仅供参考):

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1. **文献名称**:*BUB3 is essential for the assembly of a functional Mad2–Cdc20 complex in human cells*

**作者**:Taylor SS, et al.

**摘要**:本研究利用特异性BUB3抗体通过免疫共沉淀和RNA干扰技术,揭示了BUB3在纺锤体检查点中调控Mad2-Cdc20复合体形成的关键作用,并证实其缺失导致染色体分离异常。

2. **文献名称**:*Development of a monoclonal antibody against human BUB3 for cancer biomarker screening*

**作者**:Chen L, et al.

**摘要**:研究者开发了一种高特异性抗人BUB3单克隆抗体,通过免疫组化分析发现BUB3在结直肠癌组织中的表达显著上调,提示其可能作为癌症诊断的潜在生物标志物。

3. **文献名称**:*BUB3 phosphorylation regulates kinetochore-microtubule attachment in yeast*

**作者**:Hardwick KG, et al.

**摘要**:通过BUB3抗体进行Western blot和活细胞成像,发现BUB3的磷酸化修饰对其在酵母中调控微管-动粒连接的稳定性至关重要,缺陷会导致染色体错误分离。

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注:以上文献为示例,实际引用时请核实真实文献信息及摘要细节。如需具体文献,建议通过PubMed或Google Scholar检索关键词“BUB3 antibody”获取最新研究。

       

背景信息

BUB3 antibodies are essential tools for studying the spindle assembly checkpoint (SAC), a critical surveillance mechanism ensuring accurate chromosome segregation during mitosis. BUB3. a conserved SAC protein, forms complexes with BUB1 and BUBR1 to monitor microtubule-kinetochore attachment. It acts as a scaffold, facilitating protein interactions and signal transduction that delay anaphase until all chromosomes achieve proper bipolar attachment. Dysregulation of BUB3 is linked to chromosomal instability, aneuploidy, and cancer progression.

Researchers use BUB3 antibodies to investigate its expression levels, subcellular localization, and interactions in normal and pathological contexts, such as tumorigenesis or chemotherapy resistance. These antibodies are widely applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specificity validation via knockout/knockdown controls is crucial, as off-target reactivity may compromise data reliability. Recent studies also explore BUB3's non-mitotic roles, including DNA damage response and immune regulation, expanding its relevance beyond cell cycle research. Commercial BUB3 antibodies are typically raised against recombinant human protein fragments, with rabbit and mouse being common host species. Proper antibody selection and experimental optimization remain key to elucidating BUB3's multifaceted functions in health and disease.

       
关键字: BUB3抗体;BUB3;BUB3 Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业,
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,蛋白组学,细胞生物学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:重组蛋白、抗体、生物试剂
  • 公司地址:青浦区
询盘

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