**Background of VPS39 Antibody**
VPS39. also known as vacuolar protein sorting 39. is a critical component of the HOPS (Homotypic fusion and vacuole Protein Sorting) complex, which regulates membrane fusion events in lysosomal and endosomal trafficking pathways. It plays a key role in tethering late endosomes/lysosomes, facilitating autophagosome-lysosome fusion, and maintaining cellular homeostasis. Dysregulation of VPS39 is linked to lysosomal storage disorders, neurodegenerative diseases, and cancer.
Antibodies targeting VPS39 are essential tools for studying its expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate VPS39’s role in autophagy, endocytic trafficking, and cellular stress responses. Specific VPS39 antibodies help identify changes in protein levels under pathological conditions or genetic manipulations (e.g., CRISPR/Cas9 knockout).
Commercial VPS39 antibodies are typically raised in hosts like rabbits or mice, validated for specificity using knockout controls or siRNA-mediated knockdown. Their applications extend to research on diseases such as Alzheimer’s, Parkinson’s, and metabolic disorders, where lysosomal dysfunction is implicated. Reliable VPS39 antibodies thus contribute to understanding cellular quality control mechanisms and developing therapeutic strategies targeting membrane trafficking pathways.