The NDUFB3 antibody is a tool used to detect the NDUFB3 protein, a subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) in the electron transport chain. Encoded by the nuclear gene *NDUFB3*, this protein localizes to the inner mitochondrial membrane and plays a structural and functional role in Complex I assembly and activity. Complex I facilitates NADH oxidation, transferring electrons to ubiquinone while pumping protons to drive ATP synthesis. Dysregulation of Complex I subunits, including NDUFB3. is linked to mitochondrial disorders, neurodegenerative diseases, and cancer.
NDUFB3 antibodies are widely used in research to study protein expression, localization, and Complex I integrity via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They help identify mutations or deficiencies in mitochondrial disorders such as Leigh syndrome or metabolic myopathies. Additionally, studies explore NDUFB3's role in cancer metabolism, as tumor cells often exhibit altered oxidative phosphorylation. These antibodies also aid in investigating cellular responses to oxidative stress, aging, and drug targeting of mitochondrial pathways. Validated for specificity, NDUFB3 antibodies are critical in molecular and cellular biology research focused on energy metabolism and disease mechanisms.