The NUDT21 antibody is a tool used to detect NUDT21 (Nudix Hydrolase 21), also known as CFIm25. a key subunit of the cleavage factor Im (CFIm) complex involved in pre-mRNA 3'-end processing. NUDT21 plays a critical role in mRNA polyadenylation, regulating alternative polyadenylation (APA) sites to influence transcript diversity, stability, and protein expression. Dysregulation of NUDT21 has been linked to cancers, neurological disorders, and viral infections.
NUDT21 antibodies are widely employed in molecular biology research to study its expression, localization, and interactions. They are used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore NUDT21's role in APA-mediated gene regulation, cellular differentiation, and disease mechanisms. For instance, reduced NUDT21 levels correlate with shortened 3' untranslated regions (3'UTRs) in cancer-related genes, promoting oncogenesis. These antibodies also aid in identifying NUDT21's involvement in neurodevelopment and responses to cellular stress.
Commercially available NUDT21 antibodies are typically raised in rabbits or mice against specific epitopes, validated for specificity and sensitivity. Researchers rely on them to dissect post-transcriptional regulatory networks and potential therapeutic targets. Their applications extend to clinical studies, where altered NUDT21 expression serves as a biomarker for disease prognosis or drug response evaluation.