The NUDT18 antibody is a research tool designed to target the NUDT18 protein, a member of the NUDIX hydrolase family. NUDT18. also known as dATPase, is an enzyme involved in nucleotide metabolism, particularly hydrolyzing oxidized or damaged nucleotides like dATP to maintain cellular homeostasis. This function suggests its role in regulating nucleotide pools, DNA repair, and redox balance, with potential implications in cancer, aging, and neurodegenerative diseases.
The development of NUDT18 antibodies enables the study of its expression, localization, and interactions in various biological contexts. Researchers use these antibodies in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate NUDT18's tissue-specific distribution, expression levels under stress conditions, or disease states. Commercial NUDT18 antibodies are typically generated using immunogens like recombinant proteins or synthetic peptides, validated for specificity and sensitivity.
Studies employing NUDT18 antibodies have explored its regulatory roles in cell proliferation, apoptosis, and response to oxidative damage. For instance, altered NUDT18 expression has been linked to cancer progression, where dysregulated nucleotide metabolism may drive genomic instability. Reliable antibodies are critical for elucidating its molecular mechanisms and therapeutic potential. However, cross-reactivity with other NUDIX family members remains a concern, necessitating rigorous validation. Overall, NUDT18 antibodies serve as essential tools for advancing understanding of nucleotide metabolism and related pathologies.