HSP70抗体,Mouse Monoclonal HSP70 Antibody
  • HSP70抗体,Mouse Monoclonal HSP70 Antibody
  • HSP70抗体,Mouse Monoclonal HSP70 Antibody
  • HSP70抗体,Mouse Monoclonal HSP70 Antibody

HSP70抗体;HSP70 Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-25
QQ交谈 微信洽谈

产品详情

中文名称:HSP70抗体英文名称:Mouse Monoclonal HSP70 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 1424 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: HSP70
2025-05-25 HSP70抗体 Mouse Monoclonal HSP70 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 1424 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human, Monkey
IF咨询技术 Human, Monkey
IHC1/200 - 1/1000 Human, Monkey
ICC技术咨询 Human, Monkey
FCM1/200 - 1/400 Human, Monkey
Elisa1/10000 Human, Monkey
   

产品详情

AliasesRY; APG-2; HSPH2; HSPA4; hsp70RY; HEL-S-5a; HS24/P52
Entrez GeneID3308
clone2E4F10
WB Predicted band size94.3kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Monkey
ImmunogenPurified recombinant fragment of human HSP70 (AA: 642-841) expressed in Mammalian.
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using HSP70 mouse mAb against NIH/3T3 (1), Hela (2), HepG2 (3), Hek293 (4), COS-7 (5), A549 (6), and Jurkat (7) cell lysate.    


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       Flow cytometric analysis of Hela cells using HSP70 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using HSP70 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using HSP70 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using HSP70 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using HSP70 mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于HSP70抗体的代表性文献,包含标题、作者和摘要概括:

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1. **"HSP70 Antibodies in Cancer Diagnostics: A Systematic Review"**

*Author: Calderwood, S.K., et al.*

**摘要**:探讨HSP70抗体在多种癌症(如乳腺癌、前列腺癌)中的表达差异及其作为诊断标志物的潜力,强调其在肿瘤组织样本检测中的应用价值。

2. **"The Role of HSP70 in Neurodegenerative Diseases: Insights from Immunoblotting Studies"**

*Author: Dou, F., et al.*

**摘要**:通过Western blot和免疫组化分析HSP70在阿尔茨海默病模型中的表达变化,证明HSP70抗体可用于检测错误折叠蛋白的聚集及神经保护机制。

3. **"Commercial HSP70 Antibodies: Variability and Validation in Stress Response Models"**

*Author: Kampinga, H.H., et al.*

**摘要**:系统评估不同来源的HSP70抗体的特异性与灵敏度,提出标准化实验流程以提高热休克蛋白研究的可重复性。

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这些文献覆盖了HSP70抗体在疾病诊断、机制研究及实验技术优化中的应用,可作为相关研究的参考基础。如需全文链接或更多文献,可进一步提供具体研究方向。

       

背景信息

HSP70 antibodies are essential tools for studying the 70-kilodalton heat shock protein family (HSP70), a group of evolutionarily conserved molecular chaperones critical for protein homeostasis. HSP70 proteins, including stress-inducible forms like HSPA1A and constitutive isoforms like HSPA8. assist in protein folding, prevent aggregation under cellular stress, and facilitate substrate trafficking across membranes. These functions make HSP70 pivotal in diverse physiological and pathological processes, including neurodegeneration, cancer, inflammation, and immune responses.

Antibodies targeting HSP70 are widely employed in research to investigate its expression patterns, subcellular localization, and interactions. Commercial HSP70 antibodies are typically raised against specific epitopes in conserved regions, though selectivity between isoforms (e.g., distinguishing inducible vs. constitutive forms) varies depending on immunogen design. Validation remains crucial due to sequence homology among family members and cross-reactivity risks. Applications span Western blotting, immunohistochemistry, immunoprecipitation, and flow cytometry. In clinical contexts, HSP70 antibodies aid in exploring its role as a biomarker for cancer prognosis or stress responses. Recent interest focuses on its extracellular roles in immune modulation and as a potential therapeutic target. Researchers must carefully select antibodies based on target isoforms, post-translational modifications, and experimental contexts to ensure specificity.

       
关键字: HSP70抗体;HSP70;HSP70 Antibody;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
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