RHOA抗体,Mouse Monoclonal RHOA Antibody
  • RHOA抗体,Mouse Monoclonal RHOA Antibody
  • RHOA抗体,Mouse Monoclonal RHOA Antibody
  • RHOA抗体,Mouse Monoclonal RHOA Antibody

RHOA抗体;RHOA Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-25
QQ交谈 微信洽谈

产品详情

中文名称:RHOA抗体英文名称:Mouse Monoclonal RHOA Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 1446 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: RHOA
2025-05-25 RHOA抗体 Mouse Monoclonal RHOA Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 1446 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesARHA; ARH12; RHO12; EDFAOB; RHOH12
Entrez GeneID387
clone7E7H4
WB Predicted band size22kDa
Host/IsotypeMouse IgG2b
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human RHOA (AA: 1-190) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using RHOA mouse mAb against Jurlat (1), MCF-7 (2),A431 (3), and Hela (4) cell lysate.    


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       Immunofluorescence analysis of Hela cells using RHOA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using RHOA mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of HepG2 cells using RHOA mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of Jurkat cells using RHOA mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of K562 cells using RHOA mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues using RHOA mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissues using RHOA mouse mAb with DAB staining.    


           

参考文献

以下是关于RHOA抗体的3篇参考文献示例(注:内容为模拟概括,非真实文献):

1. **文献名称**:*RhoA-specific antibodies reveal the dynamics of cytoskeletal reorganization in cancer cell migration*

**作者**:Haga, R.B., et al.

**摘要**:该研究利用RHOA特异性抗体,揭示了RHOA在癌细胞迁移过程中通过调控肌动蛋白应力纤维的形成,促进肿瘤侵袭的分子机制,为靶向RhoA信号通路提供了实验依据。

2. **文献名称**:*Autoantibodies against RhoA in autoimmune diseases: Diagnostic potential and functional implications*

**作者**:Suzuki, T., et al.

**摘要**:作者发现系统性硬化症患者血清中存在RHOA自身抗体,并通过体外实验证明这些抗体可异常激活RhoA信号通路,导致成纤维细胞过度活化,提示其可能参与纤维化病理过程。

3. **文献名称**:*RhoA antibody-based inhibition of vascular smooth muscle contraction*

**作者**:Yamamoto, K., et al.

**摘要**:研究使用RHOA中和抗体阻断血管平滑肌细胞中RhoA/ROCK通路,显著抑制钙离子敏感性收缩反应,为高血压和血管痉挛性疾病提供了潜在治疗策略。

如需具体文献,建议通过PubMed或Web of Science以“RHOA antibody”为关键词检索最新研究。

       

背景信息

**Background of RHOA Antibodies**

RhoA (Ras homolog family member A) is a small GTPase protein regulating critical cellular processes like cytoskeleton dynamics, cell migration, proliferation, and gene expression. Dysregulation of RhoA signaling is implicated in cancer, autoimmune disorders, and cardiovascular diseases. RHOA antibodies are immunological tools designed to detect and study RhoA expression, localization, and activity in research or diagnostic contexts.

In cancer, RHOA mutations or altered expression are linked to tumor progression, metastasis, and drug resistance. For example, RHOA hyperactivity is observed in gastric, breast, and lung cancers. Antibodies targeting RhoA help identify its role in oncogenic pathways or tumor microenvironment interactions. In autoimmune diseases like rheumatoid arthritis, aberrant RhoA signaling contributes to inflammation and tissue damage; antibodies aid in mapping these mechanisms.

Therapeutic applications are emerging, with RHOA antibodies explored as biomarkers for disease prognosis or treatment response. Challenges include ensuring specificity due to RhoA's structural similarity to other Rho GTPases and variability in post-translational modifications. Additionally, RHOA antibodies are used in drug development, such as screening inhibitors targeting RhoA-driven pathways.

Overall, RHOA antibodies serve as vital reagents for unraveling RhoA's pathophysiological roles and advancing targeted therapies, though standardization and validation remain critical for clinical translation.

       
关键字: RHOA抗体;RHOA;RHOA Antibody;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
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