AHCYL1 (adenosylhomocysteinase-like 1) is a ubiquitously expressed protein initially identified due to its homology to S-adenosylhomocysteine hydrolase (AHCY), though it lacks enzymatic activity. It plays multifaceted roles in cellular processes, including calcium signaling, mRNA regulation, and nucleotide metabolism. AHCYL1 interacts with inositol 1.4.5-trisphosphate receptors (IP3Rs) through its binding partner IRBIT (IP3R-binding protein released with inositol 1.4.5-trisphosphate), modulating calcium release from the endoplasmic reticulum. It also participates in mRNA processing and stability by associating with RNA-binding proteins.
AHCYL1 antibodies are essential tools for studying its expression, localization, and interactions. They are widely used in techniques like Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to investigate its regulatory roles in physiological and pathological contexts. Commercially available antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, with validation in knockout cell lines to ensure specificity. Polyclonal and monoclonal formats exist, derived from hosts like rabbits or mice. Researchers utilize these antibodies to explore AHCYL1's involvement in diseases, including cancer (e.g., colorectal, liver) and neurological disorders, where its dysregulation impacts cellular proliferation, apoptosis, or metabolic pathways. Proper antibody validation remains critical due to potential cross-reactivity with homologous proteins like AHCY.