SHANK1抗体,Rabbit Polyclonal SHANK1 Antibody
  • SHANK1抗体,Rabbit Polyclonal SHANK1 Antibody
  • SHANK1抗体,Rabbit Polyclonal SHANK1 Antibody
  • SHANK1抗体,Rabbit Polyclonal SHANK1 Antibody

SHANK1抗体;SHANK1 Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-25
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产品详情

中文名称:SHANK1抗体英文名称:Rabbit Polyclonal SHANK1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 6010 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: SHANK1
2025-05-25 SHANK1抗体 Rabbit Polyclonal SHANK1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 6010 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/1000-1/2000 Human,Mouse,Rat
   

产品详情

AliasesSSTRIP; SPANK-1; synamon
WB Predicted band size225 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human SHANK1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue, Primary antibody: P11345(SHANK1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    


           

参考文献

以下是3篇关于SHANK1抗体的代表性文献及其摘要概括:

1. **"Structural analysis of the SHANK1 N-terminal region reveals a folded domain essential for synaptic function"**

*作者:Boeckers TM et al. (2005), J Biol Chem*

摘要:解析了SHANK1蛋白N端结构域的晶体结构,并利用特异性抗体验证其在突触后密度中的定位,证明该区域对突触信号传导具有关键调控作用。

2. **"Behavioral and synaptic dysfunction in Shank1 knockout mice: Implications for autism spectrum disorders"**

*作者:Silverman JL et al. (2011), Nature Neuroscience*

摘要:通过SHANK1特异性抗体检测基因敲除小鼠模型,发现SHANK1缺失导致突触可塑性受损,并引发焦虑样行为和社交缺陷,提示其与自闭症病理相关。

3. **"SHANK1 mutations in patients with autism spectrum disorder"**

*作者:Sato D et al. (2012), Human Molecular Genetics*

摘要:利用SHANK1抗体进行蛋白表达分析,鉴定出自闭症患者中新型SHANK1错义突变,证明突变导致突触蛋白复合物组装异常。

4. **"Antibody-based profiling of synaptic proteins in neurological disorders"**

*作者:Grant SGN et al. (2005), Molecular Psychiatry*

摘要:系统评估了包括SHANK1抗体在内的多种突触蛋白检测工具,建立了标准化Western Blot和免疫组化流程用于神经疾病样本分析。

注:以上文献均为模拟案例,实际引用时请通过PubMed/Google Scholar检索真实文献(关键词:SHANK1 antibody, synaptic proteins, autism)。

       

背景信息

SHANK1 antibodies are essential tools in neuroscience research, targeting the SHANK1 protein, a key member of the SHANK/ProSAP family of synaptic scaffolding proteins. SHANK1 is predominantly localized at the postsynaptic density (PSD) of excitatory synapses, where it organizes neurotransmitter receptors, ion channels, and signaling molecules by linking membrane proteins to the actin cytoskeleton. It plays a critical role in synaptic plasticity, neural development, and cognitive functions. Dysregulation of SHANK1 has been implicated in neurodevelopmental and psychiatric disorders, including autism spectrum disorders (ASD) and schizophrenia.

SHANK1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to detect protein expression, localization, and interactions. These antibodies typically recognize specific domains (e.g., ANK repeats, SH3. PDZ, or proline-rich regions) or epitopes of SHANK1. with variations in clonal specificity (monoclonal vs. polyclonal) influencing experimental outcomes. Researchers often validate SHANK1 antibodies using knockout models or knockdown controls to ensure specificity, as cross-reactivity with other SHANK family members (SHANK2. SHANK3) can occur due to structural homology.

Studies leveraging SHANK1 antibodies have advanced understanding of synaptic pathology, protein networks in neurological diseases, and potential therapeutic targets. However, discrepancies in antibody performance across studies highlight the need for rigorous validation and standardized protocols.

       
关键字: SHANK1抗体;SHANK1;SHANK1 Antibody;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
询盘

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