描述(Description)
FGF-4 Protein, Human is a member of the FGF family that transforms 3T3 cells with high efficiency, stimulates endothelial cell proliferation, migration, and protease production, and shows angiogenic activity.
生物活性(Bioactivity)
The ED50 is ≤1.5 ng/mL as measured by 3T3 cells, corresponding to a specific activity of ≥6.67 × 105 units/mg.
氨基酸序列(Target Protein Sequence)
APTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL
制剂(Trait)
Lyophilized from a 0.22 μm filtered solution of 50 mM HEPES, 750 mM NaCl, pH 7.5 or 50 mM HEPES, 750 mM NaCl, pH 7.5, 8% trehalose.
复溶(Reconstitution)
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存条件&效期(Storage & Stability)
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输方式(Shipping)
Room temperature in continental US; may vary elsewhere.

生物活性(Bioactivity)
Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.25-1 ng/mL.

电泳(SDS-PAGE)
Greater than 95% as determined by reducing SDS-PAGE.
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关键字: 科研专供;GMP级;批间一致;高纯度;生物活性;
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