描述(Description)
KGF/FGF-7 proteins coordinate embryonic development and regulate basic processes such as cell proliferation and differentiation. Its critical role extends to normal branching morphogenesis. KGF/FGF-7 Protein, Human (His) is the recombinant human-derived KGF/FGF-7 protein, expressed by E. coli , with N-His labeled tag.
生物活性(Bioactivity)
Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells.The ED50 for this effect is ≤110.3 ng/mL, corresponding to a specific activity is ≥9.07×103 U/mg.
氨基酸序列(Target Protein Sequence)
CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT
制剂(Trait)
Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 1mM EDTA, 5% Trehalose, 0.02% Tween 80, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCL, pH 7.4 or PBS or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween80 or 20 mM PB, 500 mM NaCl, pH 8.0, 8% trehalose.
复溶(Reconstitution)
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存条件&效期(Storage & Stability)
Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输方式(Shipping)
Shipping with dry ice.

生物活性(Bioactivity)
Measured in a cell proliferation assay using Mv.1.Lu Mink lung epithelial cells. The ED50 for this effect is ≤10 ng/mL.

电泳(SDS-PAGE)
Greater than 95% as determined by reducing SDS-PAGE.
特别提示:本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。
关键字: GMP级;高纯度;批间一致;生物活性;科研专供;
在生物科技领域,丰度蛋白专注重组蛋白供应,以“丰度的风帆为蛋白提供动力,丰度为蛋白护航”为使命,赋能生命科学发展。
苏州丰度依托先进技术平台,打造成熟生产体系,严控基因工程设计、细胞培养、蛋白纯化等环节,优化工艺与管理,确保产品高纯度、高活性且批间一致。产品覆盖基础研究、生物医药研发等多领域,为科研及生产提供多样、适配产品,助力突破技术难题 。