描述(Description)
Fibronectin binds collagen, fibrin, heparin, DNA, and actin. It is involved in cell adhesion, motility, opsonization, wound healing and maintenance of cell shape. GMP Fibronectin Protein, Human is a recombinant protein dimer complex containing human-derived Fibronectin protein, expressed by E. coli , with tag free. GMP Fibronectin Protein, Human, has molecular weight of ~60.0 kDa.
生物活性(Bioactivity)
The ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells has an ED50 value of 0.1-0.5 μg/mL or at 1.25 μg/ml, the efficiency of adhesion to Jurkat cells >70%.
氨基酸序列(Target Protein Sequence)
PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPS&AIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST
制剂(Trait)
Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 5.5.
复溶(Reconstitution)
It is not recommended to reconstitute to a concentration less than 100 μg/mL in injection water.
保存条件&效期(Storage & Stability)
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输方式(Shipping)
Room temperature in continental US; may vary elsewhere.

生物活性(Bioactivity)
Recombinant Human Fibronectin stimulates adhesion of B16-F1 cells. The ED50 for this effect is 35.0-420 ng/mL The activity of BY0019 was higher than other competing products.

电泳(SDS-PAGE)
Greater than 95% as determined by reducing SDS-PAGE.
特别提示:本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。
关键字: GMP级;高纯度;批间一致;生物活性;科研专供;
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