The FKBP7 antibody is a tool used to study the FK506-binding protein 7 (FKBP7), a member of the immunophilin family. FKBP7. also known as FKBP23. is a peptidyl-prolyl cis-trans isomerase (PPIase) that facilitates protein folding and interacts with molecular chaperones like calnexin in the endoplasmic reticulum. It plays roles in cellular processes such as protein trafficking, stress response, and collagen biosynthesis. Structurally, FKBP7 contains a PPIase domain and a C-terminal transmembrane domain, enabling its localization to the ER membrane.
Research highlights its involvement in embryogenesis, neural development, and cancer. For example, FKBP7 regulates hypoxia-inducible factor-1α (HIF-1α) stability in tumor microenvironments and influences extracellular matrix organization by modulating collagen secretion. Studies in model organisms, like zebrafish, suggest its importance in early development.
The FKBP7 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect expression levels, subcellular localization, and interactions. It aids in exploring FKBP7's pathological roles, including its potential as a biomarker in cancers or neurodegenerative diseases. Commercial antibodies are typically validated for specificity against recombinant FKBP7 or epitope-tagged proteins, ensuring reliability in experimental settings.