The ZBTB5 antibody is a research tool designed to detect and study the ZBTB5 protein, a member of the zinc finger and BTB/POZ (Broad-Complex, Tramtrack, and Bric-à-brac) domain-containing protein family. ZBTB5 is implicated in transcriptional regulation, potentially acting as a transcriptional repressor or activator by binding to specific DNA sequences via its C-terminal zinc finger motifs. Its N-terminal BTB/POZ domain facilitates protein-protein interactions, enabling the formation of multi-protein complexes involved in chromatin remodeling, cell differentiation, and oncogenesis. Dysregulation of ZBTB5 has been associated with cancer progression, though its precise biological roles remain under investigation.
ZBTB5 antibodies are typically produced in host species (e.g., rabbits, mice) using immunogens derived from recombinant ZBTB5 protein fragments or synthetic peptides corresponding to specific epitopes. These antibodies are validated for applications such as Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specificity is confirmed using knockout/knockdown controls or peptide blocking assays to minimize cross-reactivity with related ZBTB family members. Commercial antibodies often provide datasheets detailing batch-specific validation, recommended dilutions, and storage conditions (e.g., -20°C in glycerol-containing buffers). Researchers use ZBTB5 antibodies to explore its expression patterns, subcellular localization, and interactions with other regulatory proteins, contributing to insights into its role in development, disease, and gene regulatory networks.