The CWF19L1 antibody is a research tool designed to target the CWF19-like protein 1 (CWF19L1), a conserved eukaryotic protein implicated in cellular processes such as DNA damage response and cell cycle regulation. CWF19L1 shares homology with the yeast Cwf19 protein, which is involved in pre-mRNA splicing and genome stability. In humans, CWF19L1 is suggested to play roles in mitotic progression and chromosomal segregation, potentially interacting with components of the spindle assembly checkpoint. Dysregulation of CWF19L1 has been linked to genomic instability, a hallmark of cancer and developmental disorders.
The antibody is commonly used in molecular biology applications, including Western blotting, immunofluorescence, and immunohistochemistry, to study CWF19L1's expression, localization, and interactions. It helps researchers explore its functional relevance in diseases, particularly cancers where aberrant cell division is prevalent. Commercial CWF19L1 antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown to ensure minimal cross-reactivity.
Studies utilizing this antibody have contributed to understanding how CWF19L1 modulates checkpoint signaling and maintains genomic integrity, offering insights into therapeutic targets for cancer and genetic disorders. Its application spans basic research in cell biology and translational studies aiming to uncover biomarkers or drug candidates.