The DDX42 antibody is a research tool designed to detect DDX42. a DEAD-box RNA helicase involved in RNA metabolism, including splicing, processing, and ribosome biogenesis. DDX42 (DEAD-box helicase 42) is localized predominantly in the nucleolus and plays roles in ribosomal RNA (rRNA) maturation, stress granule dynamics, and viral RNA regulation. It has been implicated in cellular stress responses, cancer progression (e.g., leukemia, solid tumors), and interactions with viral pathogens like HIV. Antibodies targeting DDX42 are used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study its expression, subcellular localization, and interactions with RNA or protein partners. Commercial DDX42 antibodies (e.g., clones H00011325-D01P from Sigma-Aldrich, sc-365400 from Santa Cruz) are typically validated in human, mouse, or rat samples, though cross-reactivity varies. Researchers often verify specificity using knockout controls or siRNA knockdown. These antibodies have advanced studies on DDX42's role in ribosome assembly, its overexpression in malignancies, and its potential as a therapeutic target. However, batch variability and epitope masking in fixed cells remain challenges, requiring careful experimental optimization.