MACROD1 (Macro Domain Containing 1) is a protein encoded by the *MACROD1* gene, which belongs to the macrodomain family known for binding ADP-ribose metabolites. This domain is implicated in regulating ADP-ribosylation, a post-translational modification involved in DNA repair, chromatin remodeling, and cellular stress responses. MACROD1 has been studied for its role in modulating estrogen receptor-alpha (ERα) activity by removing ADP-ribose groups, thereby influencing transcriptional regulation and hormone-dependent pathways. Dysregulation of MACROD1 is linked to cancers, particularly breast and ovarian cancers, where it may act as an oncogenic driver or therapeutic target.
Antibodies targeting MACROD1 are essential tools for investigating its expression, localization, and functional interactions. They are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to study MACROD1's role in cellular processes and disease contexts. Validated MACROD1 antibodies typically exhibit specificity for human or model organism orthologs (e.g., UniProt ID: Q9BQ69) and are critical for elucidating its involvement in cancer progression, DNA damage repair, and hormone signaling. Researchers also utilize these antibodies to explore MACROD1's potential as a biomarker or its crosstalk with other ADP-ribosylation regulators, such as PARPs (poly-ADP-ribose polymerases), in therapeutic resistance mechanisms.