The WDR33 (WD repeat domain 33) antibody is a research tool designed to detect and study the WDR33 protein, a member of the WD40-repeat protein family involved in diverse cellular processes. WDR33 contains multiple WD40 domains, which facilitate protein-protein interactions and macromolecular complex assembly. It plays critical roles in RNA metabolism, including pre-mRNA 3' end processing, where it interacts with the cleavage and polyadenylation specificity factor (CPSF) complex to ensure accurate mRNA maturation. Dysregulation of WDR33 has been implicated in cancer progression, neurodevelopmental disorders, and immune responses.
Antibodies targeting WDR33 are widely used in molecular biology to investigate its expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. These antibodies help elucidate WDR33's functional mechanisms in gene expression regulation, cell cycle control, and disease pathogenesis. Commercial WDR33 antibodies are typically validated for specificity using knockout/knockdown controls and are available as polyclonal or monoclonal versions from suppliers like Abcam, Thermo Fisher, and Santa Cruz Biotechnology. Recent studies also explore its potential as a therapeutic target, particularly in cancers with aberrant RNA processing. Researchers prioritize antibody validation to minimize cross-reactivity, given the structural conservation of WD40 domains across proteins.