ATP5F1D抗体—艾普蒂

+ +

•  

   

     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P10264(ATP5F1D Antibody) at dilution 1/55. (Original magnification: ×200)    

  
  

The ATP5F1D antibody targets the ATP synthase F1 subunit delta (ATP5F1D), a crucial component of mitochondrial ATP synthase (Complex V) in the electron transport chain. ATP5F1D, encoded by the nuclear gene ATP5F1D, is part of the F1 catalytic core responsible for synthesizing ATP during oxidative phosphorylation. This subunit stabilizes the F1 complex and facilitates interactions between the catalytic α/β subunits and the rotating γ subunit, essential for converting the proton gradient into chemical energy. Dysregulation of ATP5F1D has been linked to mitochondrial disorders, metabolic syndromes, and cancers due to its role in cellular energy homeostasis.

Antibodies against ATP5F1D are widely used in research to study mitochondrial function, protein expression levels, and subcellular localization. They are applied in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate ATP synthase assembly, tissue-specific expression patterns, or mitochondrial dysfunction in disease models. Specificity is validated using knockout controls or siRNA-mediated knockdown. These tools also aid in exploring therapeutic responses in metabolic diseases or cancer, where altered ATP production is a hallmark. Commercial ATP5F1D antibodies are typically raised in hosts like rabbits or mice, available as monoclonal or polyclonal formats, and require storage at -20°C. Proper validation ensures reliability in detecting both native and denatured forms across species.